Plasmid_Backbone
pET9d-SYB1

Part:BBa_K3653013

Designed by: Shanny Ackerman, Ella Samuel   Group: iGEM20_Technion-Israel   (2020-10-12)


pET9D with Sybody 15

pET9D is bacterial expression vector with KAN antibiotic resistance and T7 promoter. Sybodies are small-sized synthetic nanobodies (camelid single-domain antibodies) with a specific and high affinity to only one cognate target. Normally they are easy to produce, stable, soluble in aqueous solutions and can refold without additional improvements. These specific Sybodies target the receptor-binding domain (RBD) of the SARS-CoV-2 spike proteins.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.


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Categories
Parameters
None